Development of 3D cell structures of pancreas as digital twins for studying nanoparticles-based therapy and diagnostic tools against PDAC (Pancreatic Ductal AdenoCarcinoma)

Department

Department of Applied Science and Technology, Politecnico of Turin

Main research activities/topics/projects

The development of three-dimensional (3D) cellular models has revolutionized the study of interactions between cells and bioactive compounds, enabling to predict with accuracy the possible response of a tissue to a new nanomedicine. 3D cell structures simulating PDAC, (i.e. Pancreatic Ductal AdenoCarcinoma) are interesting as they recapitulate the dense stroma surrounding the tumor mass and allow the creation of environments and ECM (extracellular matrix) similar to natural tissues, improving the biological relevance of in vitro experiments.

Furthermore, the advantage of these models is the avoidance of animal use in these experimentations.

The current project proposes to create and bio-engineer a 3D cell structure, with the use of hydrogels and tumor and healthy cell lines, recapitulating the complex scenario of PDAC. Once achieved, the £d models will be used to study and validate the effects of hybrid lipid NPs conceived by the TNH-LAB of prof. Valentina Cauda, which enable a simuli-responsive therapy and bio-imaging.

Working language

English

Special entry requirements

Lab experience: ability to work in sterile conditions with cell cultures, biological assays of cell viability, colorimetric cell tests, flow cytometry, fluorescence microscopy visualization and cell fixation protocols

Previous experience of preparation and characterization with nanoparticles is also welcome: for example liposomes or lipid nanoparticles, extracellular vesicles, or polymeric nanoparticles, or inorganic nanoparticles (metal/metal oxides)

Duration in months (min-max)

Phd Sanswhich: 4-9

PostDoc: 4-12