Characterizing the cellular components of the gastrointestinal (GI) tract—particularly the neuronal population—in severe dysmotility (SD) and other degenerative GI disorders is essential to understanding the extent and nature of cellular alterations. This, in turn, can improve diagnostic accuracy and help identify therapeutic targets, especially in cases where no treatment is currently available. However, to date, there is no universally recognized gold standard for evaluating these parameters.
Our objective, through the analysis of an extensive case series supported by artificial intelligence, is to identify standardized, common, and easily reproducible methods for performing these assessments on routinely processed paraffin-embedded biopsies. This approach could represent a crucial step toward a more uniform and precise evaluation of cellular alterations in the context of SD and other GI disorders.
