Attune NxT is a compact benchtop flow cytometer with hardware features that provide high resistance to blockages, reliability, and high sample flow speed. The acoustic focusing technology allows for sample flow speeds ranging from 12.5 to 1,000 µL/min (up to 35,000 events/sec with all parameters), approximately 10 times faster than traditional cytometers, without compromising data integrity.
It is equipped with a 488 nm solid-state laser (4 colors; 6 parameters).
The alignment is fixed and achieved with a pre-aligned welded fiber, eliminating the need for user maintenance.
Potential applications include:
Evaluation of protein expression of surface, cytoplasmic, and intranuclear cellular markers on live or fixed cells
Evaluation of transfection efficiency
Assessment of post-translational modifications of proteins (e.g., phosphorylation)
Evaluation of RNA: IncRNA, miRNA, and mRNA transcripts
Assessment of cell viability: apoptosis/necrosis
Study of the cell cycle and polyploidy
Evaluation of cellular growth kinetics in populations
Identification and characterization of hematological cell subpopulations
For the use of the instrument for services and/or potential scientific collaborations, please contact:
TECHNICAL SPECIFICATIONS:
1 Blue solid-state laser (488 nm) (4 colors; 6 parameters).
10 x 50 μm flat-top laser for optimized alignment (Coefficient of Variation (CV) < 3%).
Acoustic focusing system for high acquisition speeds.
Forward scatter: photodiode detector with a 488/10 nm bandpass filter.
Side scatter: PMT with pre-defined 488/10 nm bandpass filter; optional 405/10 nm bandpass filter.
Attune Cytometric Software: Data is acquired and saved in atx format, but also in FCS (a format readable by most common analysis programs, e.g., Flowjo).
Compensation: complete matrix with both automated and manual modes.
